For rotifer culture two easy reefsĀ® microalgae species are used: Nannochloropsis (80%) +Tetraselmis (20%). The dosage will depend on the number of rotifers reached. The average consumption is of 4 ml Easyroti per litre of rotifer culture per day (25% of rotifer will be harvested every day).
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To keep a rotifer culture follow the directions below:
Material needed:
3 cylinder flasks of 2 L with slow bubbling.
Rotifer inoculums.
Food needed:
easy reefsĀ® Nannochloropsis (80%) +Tetraselmis (20%)
Culture cycle from strain: 5 days
Recommended sea water density: 1019, although they can be cultured well up to 1022.
Culture temperature: 22ĀŗC to 28ĀŗC. Optimum culture temperature: 24ĀŗC.
To filter the rotifer you should use a 50 micron net.
Once you start the culture you will be able to harvest rotifers after 6 days. You will have one flask for the āstrainā and another one for the āproductionā culture.